DSG-Ⅰ型生物信息紅外肝病治療儀治療大鼠非酒精性脂肪肝

   世界華人消化雜志 2008年3月28日16(9):935-940 ISSN1009-3079 CN14-1260/R
                                                                          •論著•
竇愛霞，宗蕾，周琨，陳婷，潘浩，張權，李繼強，房靜遠，陸倫根
竇愛霞, 宗蕾，周琨，陳婷，潘浩，張權，李繼強，房靜遠，陸倫根,上海交通大學醫學院附屬仁濟醫院消化內科  上海市消化疾病研究所 上海市  200001
竇愛霞，2005級上海交通大學醫學院附屬仁濟醫院消化內科碩士，主要從事非酒精性脂肪性肝病發病機制及治療的研究。
上海市科學技術委員會資助項目，No.054119618
上海市重點學科建設資助項目，No.Y0205
作者貢獻分布：陸倫根、竇愛霞、潘浩對此文所作貢獻均等；此課題由陸倫根、竇愛霞、張權、李繼強及房靜遠設計；研究過程由竇愛霞、宗蕾、周琨、陳婷及潘浩操作完成；數據分析由宗蕾、潘浩完成；本論文寫作由竇愛霞及陸倫根完成
通訊作者：陸倫根,200001,上海交通大學醫學院附屬仁濟醫院消化內科，上海市消化疾病研究所.lungenlu1965@yahoo.com
電話：021-53882001           傳真：021-63266027
收稿日期：2007-09-16         修回日期：2008-03-08
Experimental study on nonalcoholic fatty liver disease treated by medical instrument on hepatic diseases of type DSG in rats
Ai-Xia Dou, Lei Zong,Kun Zhou,Ting Chen,Hao Pan，Quan Zhang，Ji-Qiang Li， Jing-Yuan Fang，Lun-Gen Lu

Ai-Xia Dou, Lei Zong, Kun Zhou, Ting Chen, Hao Pan，Quan Zhang，Ji-Qiang Li， Jing-Yuan Fang，Lun-Gen Lu. Department of Gastroenterology，Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai JiaoTong University School of Medicine， Shanghai 200001 ,China
Supported by: the science Technology Committee Program of Shanghai, No.054119618, the Shanghai Leading Academic Discipline Project No.Y0205
Correspondence to:Dr. Lun-Gen Lu, Department of Gastroenterology，Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai JiaoTong University School of Medicine，Shanghai 200001 China. lungenlu1965@yahoo.com
Received:2007-09-16   Revised:2008-03-08
Abstract
AIM: To investigate the primary therapeutic efficacy of  the medical instrument on hepatic
diseases of type  I  DSG in treating  nonalcoholic fatty liver disease (NAFLD）of rats．
METHODS: Fifty male SD rats were randomly divided into 5 groups.The control groups (n=20,N1 and N2 group) were fed with normal diet; and the model groups (n=30,M1,M2 and T group) were fed with fat-rich diet consisting of 0.88 standard normal diet+0.10 lard oil +0.02 cholesterol.Therapeutic group (T group) was treated from wk 13 and finished at the wk 20..The  levels of serum alanine aminotransferase (ALT)、aspartate aminotransferase (AST)、gamma-glu-tamyl-transpeptidase (GGT)、alkaline phospha-tase (ALP)、total protein (TP)、triglycerides (TG)、total cholesterol (TC)、low-density lipoprotein (LDL)、and high density lipoprotein (HDL) were measured. The hepatic histologic changes were observed with HE and Masson staining.
RESULTS： At the end of the experiment,compared with those in the normal control group，the liver index (t=8.65,p<0.01)and serum ALT(t=4.65,p<0.01)、AST(t=4.74,p<0.01)、ALP(t=3.56,p<0.01)、TP(t=6.35,p<0.01)、TC(t=8.41,p<0.01)、LDL(t=8.86,p<0.01) levels were increased significantly in the model control group，and the hepatic steatosis (z=-4.135,p<0.01)、inflammation(z=-3.752,p<0.01) and fibrosis (z=-4.156,p<0.01)were more serious. Compared with those in the model control groups，the serum ALT、GGT、ALP、TP、TG、and TC levels were all down-regulated in the therapeutic group without significances, while AST(t=-2.404,p<0.05) level was reduced significantly.The degrees of the hepatic steatosis and inflammation were decreased without statistical significances,but fibrosis degrees were lightened markedly(z=-3.101,p<0.01).
CONCLUSION: The medical instrument on hepatic diseases of type I DSG has some therapeutic effect in treating NAFLD of rats.
Key Words:  Nonalcoholic fatty liver disease; Medical instrument on hepatic diseases of type IDSG ;Rat
Dou AX, Zong L,Zhou K, Chen T ,Pan H, Zhang Q,Li JQ,Fang JY,Lu LG.Experimental study cinnonalcoholic fatty liver disease treated by medical instrument on hepatic diseases of type I DSG in rats .Shijie Huaren Xiaohua Zazhi 2008;16(9):935-940
摘要
目的：初步觀察DSG-Ⅰ型生物信息紅外肝病治療儀治療大鼠非酒精性脂肪性肝病的療效。
方法：♂SD大鼠50只隨機分組，正常對照組20只喂飼普通飼料，模型對照組及近紅外光治療組共30只喂飼高脂飼料（0.88標準普通飼料＋0.10豬油＋0.02膽固醇）。治療組自13wk始進行近紅外線照射治療，于20wk結束治療并處死各組大鼠，測定血清ALT、AST、GGT、ALP、TP、TG、TC、LDL和HDL水平，同時觀察肝組織學改變。
結果：20wk試驗結束時，與正常對照組相比，模型對照組肝指數(t=8.65,p<0.01)、血清ALT(t=4.65,p<0.01)、AST(t=4.74,p<0.01)、ALP(t=3.56,p<0.01)、TP(t=6.35,p<0.01)、TC(t=8.41,p<0.01)、LDL(t=8.86,p<0.01) 水平顯著升高，肝組織脂肪變性(z=-4.135,p<0.01)、
炎癥(z=-3.752,p<0.01) 及纖維化程度 (z=-4.156,p<0.01)均較對照組程度重。與模型對照組相比，近紅外光治療組肝指數、血清ALT、GGT、ALP、TP、TG和TC水平均有所下降，但無統計學意義，而AST有明顯降低(t=-2.404,p<0.05)；肝組織脂肪變及炎癥程度較模型組輕，但無統計學意義，而肝纖維化程度較模型組減輕(z=-3.101,p<0.01)。
結論：DSG-Ⅰ型生物信息紅外肝病治療儀對大鼠高脂飲食非酒精性脂肪性肝病有一定的治療作用。
關鍵詞：非酒精性脂肪性肝病；DSG-Ⅰ型生物信息紅外肝病治療儀；大鼠
竇愛霞, 宗蕾，周琨，陳婷，潘浩，張權，李繼強，房靜遠，陸倫根
DSG-Ⅰ型生物信息紅外肝病治療儀大鼠非酒精性脂肪肝.世界華人消化雜志2008;16(9)935-940
http://www.wjgnet.com/1009-3079/16/935.asp